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Fig. 2. Cytokinesis defects in C. elegans embryos derived from mothers of the genotype unc-45(st601let) /unc-45(r450ts) carrying an extrachromosomal array with the wild-type unc-45 cDNA fused to GFP, raised at 25°C. (A-E) Time course shows the same embryo photographed at different times. Complete cytokinesis was not observed, although many attempts were made. Times are relative to the beginning of observation: (A) 0 minutes; (B) 15 minutes; (C) 25 minutes; (D) 40 minutes; (E) 70 minutes. (F) Embryo from a different mother of the same genotype. Note the multiple nuclei in one cell (arrowhead). (G-I) A wild-type embryo is normally able to complete at least two cell divisions in a similar time. (G) 0 minutes, pronuclear meeting; (H) 4 minutes, first cleavage; (I) 14 minutes, second cleavage. Embryos were observed under differential interference contrast optics.





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