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Fig. 4. Immunolocalization of UNC-45 and NMY-2 in wild-type and nmy-2(RNAi) embryos. (A-F) Wild-type embryos of increasing age stained with anti-UNC-45 (green) and anti-NMY-2 (red) to show the concentration of UNC-45 and NMY-2 at the cell boundaries. (A-C) Two-cell embryo with the merged image showing colocalization of UNC-45 and NMY-2. (D-F) ~20-cell stage embryo with merged image. Images G and H show embryos treated with RNA-mediated interference (RNAi) against NMY-2, stained with anti-UNC-45 showing that the concentration of UNC-45 at the cell boundaries is disrupted when NMY-2 is knocked down.





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