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Fig. 4. Treatment of digitonin-permeabilised cells with fluorescently labelled thymosin ß4. (A-A'') HeLa cells were permeabilised with digitonin and thereafter incubated with fluorescently labelled thymosin ß4 (A') and the chromatin specific dye chromomycin (A) with the merged image shown in A''. Note the pure cytoplasmic distribution of thymosin ß4. (B-B'') HeLa cells were permeabilised with digitonin and further treated using 0.2% Triton X-100. (B) chromomycin staining, (B') labelled thymosin ß4, (B'') merged image. Bar, 50 µm.





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