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Fig. 3. PC-3 cells stably expressing BENE-myc or MAL-GFP. Cells were fixed and stained with MAL (A), MAL2 (B,D) and BENE (C) and analyzed with a confocal laser microscope. PC-3 cells stained with cav-1 were analyzed by immunofluorescence microscopy (E) and by phase-contrast microscopy (F). PC-3 cells stably expressing MAL-GFP were analyzed by immunofluorescence microscopy (G) and by phase-contrast microscopy (H). (I) Electron micrograph of PC-3 cells embedded in Epon. Cells in A-D have been outlined for better visualization. Bars A-H, 10 µm; I, 1 µm.