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Fig. 4. Confocal immunofluorescence studies of cav-1. (A-F) PC-3 cells stably expressing BENE-myc were fixed and double-labelled with antibodies to cav-1 and the GPI-anchored protein CD59, and cav-1 and transferrin receptor (Tfr). (G-I) In some experiments, PC-3 cells were previously incubated with BFA (2.5 µg/ml) for 30 minutes, fixed and then double-labelled with antibodies to cav-1 and transferrin receptor. Cells have been outlined for better visualization. Bars, 10 µm.