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Fig. 7. Cav-1 and MAL-GFP secretion in prostasomes continues in the presence of BFA but it is reduced by wortmannin. (A-D) PC-3 cells stably expressing BENE-myc or MAL-GFP were incubated without or with BFA (2.5 µg/ml) (A,B) and without or with wortmannin (wort.) (100 nM) (C,D) in serum-free medium for 20 hours. Prostasomes were then isolated from the culture supernatant and analyzed by western blotting using antibodies to cav-1 and GFP. A and C show a representative experiment and a quantitative analysis of cav-1 secretion from three experiments. (E) PC-3 cells were treated with wortmannin (100 nM) for 1 hour and then fixed and double-labelled with antibodies to cav-1 and the endosomal marker Rab5. Bar, 10 µm.
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