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Fig. 2. Local dynamics at DNA replication sites in living HeLa cells. (a) Enlargements of two replication sites associated with the nucleolar (N) periphery (labelled with small arrows in Fig. 1) at the indicated time points (minutes after microinjection). GFP and Cy3 fluorescence and the corresponding merged images are shown (green, GFP; red, Cy3). At 95 minutes the nascent DNA synthesized at the two GFP-labelled sites is translocated between these two sites (arrow). At 120 minutes intense GFP fluorescence appears at a site previously devoid of GFP-PCNA (arrowhead), adjacent to a previous site of synthesis where the nascent DNA is still associated with the nucleolar periphery. (b) Fluorescence intensity profiles along the lines indicated on the merged images in a. The x-axis indicates the path of the line measured in µm with 0 corresponding to the left end of the line. Arrow and arrowhead point to the same regions as in a. Bars, 1 µm.





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