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Fig. 5. Spatial dynamics of GFP-PCNA at sites harbouring labelled DNA foci. (a) Light optical section of a nucleus labelled according to the scheme depicted in Fig. 4a at the beginning of the imaging period (red, Cy3 fluorescence; green, GFP fluorescence; yellow, colocalization). The arrow points to a region shown enlarged in b-d. (b-d) Time series (indicated in minutes) of the nuclear sub-region marked with an arrow in a. The arrow in b points to a Cy3-labelled focus that colocalized with a GFP-PCNA focus at the beginning of the imaging period. At later time points (c,d), the GFP fluorescence appeared in an adjacent region (arrowhead) between this and a neighbouring Cy3-labelled focus. Bar in a, 5 µm; b-d, 1 µm.





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