spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 7. Spatial arrangements of GFP-PCNA and Cy3-labelled foci in class I and class III nuclei. Nuclei were labelled according to the experimental procedure outlined in Fig. 4a. GFP and Cy3 fluorescence and the corresponding merged images (green, GFP; red, Cy3; yellow, colocalization) are shown. (a) Single light optical sections of a class I nucleus. The upper panels show the nuclear periphery whereas the lower panels show an equatorial plane. GFP-PCNA foci and labelled DNA foci localize adjacent to each other. (b) Single light optical sections showing an equatorial plane of a class III nucleus. The nucleus displays a pattern typical for early S phase with many foci distributed throughout the nuclear interior. Colocalization at the level of individual foci becomes evident in regions relatively devoid of labelled foci (arrows). However, colocalization at the level of individual foci can also be observed in more crowded regions (arrowheads). Bars, 10 µm.





Right arrow Return to article