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Fig. 8. Spatiotemporal interaction of the replication machinery with adjacent sets of DNA foci. (a) The panels show light optical sections from the equatorial planes of two sister nuclei labelled and imaged according to the experimental procedure outlined in Fig. 4a. Arrows point to regions with labelled foci shown enlarged in b. The panels show single GFP, Cy3 and Cy5 fluorescence with merged image as indicated (green, GFP; red, Cy3; blue, Cy5; red and green appears yellow; red and blue appears violet; a merge of all three colours appears white). The number of GFP foci corresponds to the number of Cy3-labelled foci. (b) GFP-labelled foci colocalize with Cy3 but not with adjacent Cy5-labelled foci. Bar in a, 10 µm; b, 1 µm.





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