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Fig. 4. Interaction of the 5-HT_4(a)R variant with both SNX27a and SNX27b in a PDZ-dependent manner. HEK-293 cells were co-transfected with the indicated cMyc-tagged-5-HT₄R, with or without HA-tagged-SNX27 constructs using Lipofectamine^TM 2000. 24 hours after transfection, total cell lysates were immunoprecipitated with anti-Myc cross-linked protein-A/Sepharose. Immunoprecipitated proteins were resolved by SDS-PAGE and detected by immunoblotting using the anti-HA antibody (top). Input (bottom) represents 5% of the total protein used in immunoprecipitation. The data illustrated are representative of three experiments.

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