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Fig. 1. Genomic locus of mCx39 containing the unspliced open reading frame. Predicted transmembrane regions (HUSAR subprogram Tmhmm) are underlined and the boxed peptide sequences represent the epitopes to which antisera were raised. Conserved cysteine residues are encircled. Underlined nucleotide sequences represent (from upstream to downstream) the Kozak-consensus motif of translational initiation, both the splice-donor and acceptor sites and the canonical motifs for transcriptional termination. Conserved nucleotides are shown in bold, nucleotide stretches rich in pyrimidines are italic and the branch point of splicing is boxed. The sequence of the 
1.5 kb intron between exons 1 and 2 is largely excluded. After splicing, both shaded glycine residues are replaced by one glycine encoded by the restored triplet. Nucleotides and amino acids in bold italics represent the following exchanges found between the 129Sv-derived mCx39 sequence (AJ414562) and the C57BL/6-derived version: nucleotide 2291 G to A; 2546 T to C; 2580 G to T; leading to a Gly to Cys substitution at nucleotide 345 and C to T substitution at nucleotide 2613. The genomic structure of mouse and human connexin genes whose coding region is restored after functional splicing as illustrated in the diagram.
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