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Fig. 5. Immunoblot analysis of mCx39-transfected HeLa cells and different mouse tissues. (A) Two signals at about 40 kDa and 80 kDa were prominent in mCx39-transfected HeLa cells but not in wild-type cells. (B-D) Expression of Cx39 in different mouse tissues at different stages of development. At ED16.5 (B) and P0 (C), both signals at 40 and 80 kDa could be readily detected but were absent after probing various adult mouse tissues (D). Thus, the 80 kDa protein band is suspected to be the dimeric form of Cx39.





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