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Fig. 8. Immunolocalization of Cx39 in intercostal muscle and diaphragm of embryonic mice. (A-C) 10 µm cryosections of embryonic (ED14.5) intercostal muscle (A) and diaphragm (B,C) were incubated with rabbit anti-Cx39 and Alexa488-conjugated goat anti-rabbit antibodies. The punctate green staining pattern suggests that the expression of Cx39 at ED14.5 occurs only in primary myotubes. (C) Diaphragm after incubation without anti-Cx39 antibodies. Bar, 20 µm.





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