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Fig. 1. Development of cultures emanating from individual mouse myofibers. (A-E') Myofibers labeled with DAPI (blue) and anti-Pax7 (red); fibers were fixed at time 0 (A and A'), 19 hours (B and B'), 24 hours (C and C'), 48 hours (D and D') and 72 hours (E and E'). (F-F'') Parallel images of 1-week-old myofiber culture labeled with DAPI, anti-Pax7 and anti-MyoD, respectively; image of each stain was merged with the phase-contrast image; arrow points to a cell negative for both Pax7 and MyoD. (G-H') Parallel DAPI-stained and phase-contrast images of two different areas within the same 4-week-old culture, depicting adipocytes (G and G') and myotubes (H and H'); nuclei in the adipogenic area are larger then the myonuclei. Bar, 20 µm (A-F''); 90 µm (G-H').





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