spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Inhibition of MK-induced neurite outgrowth by anti-{alpha}6-integrin antibody. Plastic 24-well culture plates were coated with 10 µg ml–1 of MK for 2 hours at room temperature. Then, a grid pattern was made by the ultraviolet-inactivation technique using electron-microscopy grids. After blocking with 10 mg ml–1 BSA, brain cells (1x106) from mouse embryonic cerebral cortex were cultured without further addition (A) or with 0.0045% NaN3 (B), 50 µg ml–1 anti-{alpha}4-integrin antibody (C) or anti-{alpha}6-integrin antibody (D) at 37°C under an atmosphere containing 5% CO2. (E) Effects of anti-{alpha}6-integrin antibody on neurite outgrowth on wells coated with 20 µg ml–1 MK. The proportion of neurons with a defined neurite length was determined by enumerating cells in ten fields at 400x magnification. The average value obtained in three different wells is shown with the s.d. Phase-contrast photomicrographs were taken after culture for 48 hours using a Nikon DIAPHOTO and Olympus CCD camera C5530. Scale bar, 50 µm.





Right arrow Return to article