|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 6. Increased rate of degradation of GLT-1 in G93A-expressing MDCK cell lines. (A) Forty-eight hours after GLT-1 transfection, the indicated cell lines were treated for 6 hours with 60 µg/ml cycloheximide (CHX) to inhibit protein synthesis or overnight with 100 µM chloroquine (CHQ) to inhibit lysosomal degradation. Equal amounts of proteins were separated by 10% SDS-PAGE and immunoblotted for GLT-1. (B) The pixel densities of the lanes from the experiment shown in A were evaluated using the NIH Image program. The values were expressed as fold increases in protein expression in treated compared to untreated cells. When protein synthesis was inhibited by cycloheximide, GLT-1 levels were reduced to 18% in G cells, whereas a negligible reduction in GLT-1 was observed in M or S cells. Chloroquine treatment protected GLT-1 from lysosomal degradation mainly in G cells.
|
