|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 1. Insulin and PDGF increase the amount of GLUT4myc at the cell surface and stimulate glucose uptake with different time courses that correlate with Akt phosphorylation. Confluent GLUT4myc myoblasts were incubated with either 100 nM insulin or 50 ng ml–1 PDGF-BB at 37°C for the times indicated, and assayed for (A) GLUT4myc translocation or (B) 2-deoxyglucose uptake; *, P<0.05 against time 0. The means±s.e.m. of at least three independent experiments are shown per condition, each done in triplicate. (C) Total cell lysates were separated by 10% SDS-PAGE and immunoblotted with anti-phospho-Akt (Ser 473) antibody. The same membrane was stripped and reblotted for total Akt.
|
