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Fig. 5. Tetanus toxin (TeTx) reduces the insulin-dependent, but not the PDGF-dependent, gain in GLUT4myc at the surface. GLUT4myc myoblasts were co-transfected with 0.6 µg of eGFP-encoding cDNA and 0.9 µg of either pcDNA3 (empty vector) or TeTx-encoding cDNA, as indicated. (A) After 24 hours, cells were serum starved and then stimulated without insulin (a,b), with 100 nM insulin (c,d) or with 50 ng ml–1 PDGF (e,f). Cells co-expressing TeTx and eGFP are shown at the top, and the corresponding anti-Myc surface staining from the same field of view is shown at the bottom. All images are collapsed xy projections. (B) Quantification of cell-surface GLUT4myc labeling in empty vector and TeTx-transfected cells, showing the means±s.e.m. of three independent experiments. Scale bar, 10 µm.
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