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First published online October 27, 2004

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Imaging adhesion – the 4^th dimension

Cell migration is a cyclic process in which integrin-containing adhesion complexes continually turn over: these assemble at the front of the cell as protrusions form and disassemble at the rear when it retracts. To understand the macromolecular choreography involved, we need new, quantitative methods to follow it. On p. 5521, Claire Brown and co-workers describe how they have developed image correlation microscopy (ICM) to do just this. Previously used only for static images, ICM is a variant of fluorescence correlation spectroscopy (FCS) – a technique for determining the concentration, aggregation state and dynamics of fluorescently tagged molecules. Brown and co-workers have now extended the technology to allow them to analyse image stacks from time-lapse sequences retrospectively and dynamically map adhesion turnover. This approach reveals that integrin 5 is present in clusters before it enters adhesions but becomes further clustered in nascent adhesions, whose formation it appears to initiate. It also demonstrates that 5 and the cytoskeletal linker -actinin are robustly associated even outside adhesion complexes. Finally, the new technique shows that, as adhesions disassemble, whereas paxillin diffuses away rapidly, integrins often remain attached to the substrate and -actinin is transported away by some form of retrograde flow.

Related articles in JCS:

Spatial mapping of integrin interactions and dynamics during cell migration by Image Correlation Microscopy

Paul W. Wiseman, Claire M. Brown, Donna J. Webb, Benedict Hebert, Natalie L. Johnson, Jeff A. Squier, Mark H. Ellisman, and A. F. Horwitz
JCS 2004 117: 5521-5534. [Abstract] [Full Text]  

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Related articles in JCS Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Search for Related Content