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Fig. 1. Recruitment of PH-Akt-GFP to the leading edge of the cell during chemotaxis. A DNA vector expressing PH-Akt-GFP was transiently transfected into HEK293 cells stably expressing either (A) WT CXCR2 or (B) LLKIL mutant CXCR2. Time-lapse fluorescence microscopic images were taken every minute immediately after a point source of ligand (1 µg/ml MIP-2) was applied. The number in each picture shows the time-lapse in minutes and the arrow indicates the direction of ligand application. The arrowheads point towards the accumulation of PH-Akt-GFP. Scale bars, 20 µm. Each experiment was repeated at least three times and representative images are shown. The time-lapse video clips are available online (Movies 1 and 2 in supplementary material).
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