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Fig. 2. Akt activity is required for CXCR2-mediated chemotaxis. HEK293 cells stably expressing WT CXCR2 were transiently transfected with either dominant negative Akt (black bars) or vector control (open bars). The chemotaxis assays were performed in a modified Boyden chamber. Duplicates were used for each sample and the number of cells in ten fields was counted for each well. The chemotaxis index was calculated as described in Materials and Methods. Error bars indicate the s.e.m. ANOVA analysis was performed to evaluate the statistical difference between vector control and dominant negative Akt (P<0.01).
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