|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 3. Inhibition of CXCR2 internalization by dominant negative dynamin results in inhibition of PH-Akt recruitment. HEK 293 cells stably expressing WT CXCR2 were co-transfected with PH-Akt-GFP, and either (A) wild-type dynamin or (B) dominant negative dynamin (K44A). Time-lapsed fluorescence microscopy was carried out for each group of transfected cells. Point source of ligand (MIP-2) was given as described in Materials and Methods and the arrow indicates the direction of ligand application. Scale bars, 20 µm. The number inside each picture gives the time-lapse in minutes after initiation of ligand stimulation.
|
