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Fig. 6. Activation of Cdc42 and Erk1/2 in HEK293 cells expressing either WT or LLKIL mutant receptors upon agonist stimulation. HEK 293 cells stably expressing either WT CXCR2 or LLKIL mutant receptor were treated without or with MIP-2 (100 ng/ml) for 5 minutes. The affinity precipitation assay (see Materials and Methods for details) was performed for Cdc42 activity and western blots with an p-Erk1/2-specific antibody was performed for the Erk1/2 activation test. Experiments were repeated at least three times with similar results. (A) A representative western blot from three experiments. (B) Quantification of the western blots with error bars for s.e.m. Significant differences of ligand treatment versus non-treatment are indicated by asterisks (P<0.05, Student's t-test).
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