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Fig. 7. The inhibition of dominant negative and constitutively active Cdc42 on chemotaxis in HEK 293 cells. HEK 293 cells stably expressing WT CXCR2 were transfected with pCDNA3 vector (control, black bars) or dominant negative Cdc42 (DN-Cdc42, gray bars) or constitutively active Cdc42 (CA-Cdc42, open bars) constructs. Chemotaxis assays were performed 48 hours after transfection. Each assay was repeated at least three times. Error bars give s.e.m. An ANOVA was performed for the comparison between vector control and either dominant negative or constitutive active Cdc42 (P<0.01).





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