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Fig. 4. Characterization of the poles in monopolar and bipolar figures after 23C-
-tubulin depletion. (A) Chromosomes appear in blue and microtubules in red, and the staining in green corresponds to the polar mitotic markers Asp (a,b,g,h), CP190 (c,d,i,j) or centrosomin (e,f,k,l). (Aa-f) Monopolar figures. Asp labelling was always exclusively localized to the single pole in several dots corresponding in most cases to sub-spindles (a,b). CP190 labelling was concentrated at the pole as between one and four dots (d). Centrosomin (CNN) was also concentrated at the pole, usually as a large and irregular structure (e,f). (Ag-l) Bipolar figures. Asp was always localized to the two poles, where, in most cases, it constituted a large area composed of two to five dots associated with each sub-spindle (g,h). CP190 and centrosomin labelling were present as one (i) or several (k) dots located at both poles or at one pole (j,l). (B) Renucleation in -tubulin-depleted cells. Microtubule arrays (red) renucleated from one or two centrosomin-labelled spots (green) after cold depolymerization. Scale bar, 5 µm.
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