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Fig. 5. VP8 treatment does not alter the protein content of the tight junction (TJ) proteins ZO-1, occludin and claudin-3, but modifies the distribution pattern observed by immunofluorescence. (A) Confluent MDCK monolayers were incubated in media with 4 µg/ml of GST or GST-VP8 or kept in control media. After one hour, the monolayers were fixed and processed for immunofluorescence. Bar, 40 µm. (B) Western blot detection of ZO-1, claudin-3 and occludin in the Triton X-100 soluble and insoluble fractions, obtained from monolayers cultured in control media, treated for 1 hour with 4 µg/ml of GST or GST-VP8.





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