JCS01416 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. Examples of the various calculated correlation functions. (A) Spatial autocorrelation function from a5-GFP expressed in a CHO B2 cell. The front facing quadrant shows a fit of the data to Eqn 14. (B) Sample temporal correlation function from an image time series of a cell expressing a-actinin-GFP. Temporal correlation plots show diffusion (C, Eqn 11), a single population that is diffusing and flowing (D, Eqn 12), diffusion with a significant immobile population (E, Eqn 11), and two populations one diffusing and one flowing (F, Eqn 13). (G) Spatial-temporal correlation functions for a simulation of a flowing protein used in the velocity mapping analysis. Data are shown for a simulation and shows the velocity correlation peak first distorting the central correlation peak and then splitting off from the central peak. The direction and distance for the velocity peak is used to calculate the direction and magnitude of directed motions.

• Movie 1 -

  Movie 1. CHO B2 cell expressing a5-GFP. Movie frames were collected on an Olympus Confocal using 0.2% power from the 488 nm laser line of a 40 mW Ar ion laser in the slow scan mode (see above). Pixel resolution is 0.111 mm and time resolution is 10 seconds per frame.

• Movie 2 -

  Movie 2. CHO K1 cell expressing a-actinin-GFP. Movie frames were collected on a two-photon microscope (see above). Pixel resolution is 0.118 mm and time resolution is 5 seconds per frame.

• Movie 3 -

  Movie 3. CHO B2 cell expressing a5-GFP. Movie frames were collected on an Olympus Confocal as for Movie 1. Pixel resolution is 0.111 mm and time resolution is 10 seconds per frame.