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Fig. 7. Temporal ICCM Correlation Functions for {alpha}5-integrin-YFP correlated with {alpha}-actinin-CFP or paxillin-CFP. Temporal ICCM correlation functions were calculated from two-photon microscope image time series of CHO B2 cells expressing {alpha}5-integrin-YFP and {alpha}-actinin-CFP (A) or {alpha}5-integrin-YFP and paxillin-CFP (B). The integrin-paxillin data were calculated from images in a central region of the cell where there was no organized paxillin. The temporal integrin autocorrelation function (closed circles), {alpha}-actinin or paxillin autocorrelation function (open triangles) and the cross-correlation function (closed squares) are shown. Cross-correlation functions and the YFP autocorrelation function were corrected for bleed through of the CFP signal into the YFP channel (see Materials and Methods).





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