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Fig. 3. Cellular localization of recombinantly and endogenously expressed BCR. (A) BHK-21 cells transiently expressing Bcr were immunostained with rabbit anti-Bcr and mouse anti-plasma membrane calcium ATPase (PMCA-ATPase) antibodies as described in Materials and methods and viewed by confocal microscopy. (B) Endogenous Bcr, ß-COP and clathrin were visualized in HEK293 cells by immunofluorescence microscopy using rabbit anti-Bcr and mouse anti-ß-COP or mouse anti-clathrin heavy chain antibodies. (C) Endogenous Bcr and CFTR were detected by immunofluorescence staining in polarized Calu-3 cells, grown at an air-liquid interface using rabbit anti-Bcr and mouse anti-CFTR antibodies.





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