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Fig. 5. Confirmation of the Bcr-Mint3 interaction. (A) Mint3 and Bcr-GST were transiently expressed in BHK-21 cells. Lysates prepared from these cells were incubated with glutathione-Sepharose beads and bound products were separated by SDS-PAGE. Mint3 was detected by western blotting using the rabbit anti-Mint3 antibody. The control is from untransfected parental BHK cells. (B) Similar experiment employing Bcr with deletion of 3 C-terminal amino acids. (C) Mint3 is detected in an immunoprecipitate with a Bcr antibody in lysate from parental HEK293 cells. Controls using an irrelevant antibody or immunoblotting with only secondary antibody, were negative for this band.
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