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Fig. 6. Tip1p and Ssm4p collaborate to localise Dhc1p. (A) Localisation of Ssm4p-GFP in the dhc1 and tip1 mutant zygotes. (B) Localisation of GFP-Dhc1p in the ssm4 ${Delta}$ , tip1 ${Delta}$ and ssm4 ${Delta}$ tip1 ${Delta}$ mutant zygotes. (C) cyr1 ${Delta}$ sxa2 ${Delta}$ dhc1GFP, cyr1 ${Delta}$ sxa2 ${Delta}$ ssm4 ${Delta}$ dhc1GFP, cyr1 ${Delta}$ sxa2 ${Delta}$ tip1 ${Delta}$ dhc1GFP, cyr1 ${Delta}$ sxa2 ${Delta}$ tip1 ${Delta}$ ssm4 ${Delta}$ dhc1GFP cells were grown in pheromone for 6 hours and then imaged with a fluorescence microscope. The white arrows indicate the microtubule tips and the yellow arrowheads the SPB. (D) cyr1 ${Delta}$ sxa2 ${Delta}$ ssm4 ${Delta}$ dhc1GFP, cyr1 ${Delta}$ sxa2 ${Delta}$ tip1 ${Delta}$ ssm4 ${Delta}$ dhc1GFP cells were grown in pheromone for 6 hours and then fixed and stained for tubulin. (E) ssm4 ${Delta}$ dhc1GFP, tip1 ${Delta}$ ssm4 ${Delta}$ dhc1GFP zygotes were fixed and stained for tubulin. (F) Colocalisation of Tip1p and Dhc1p. cyr1 ${Delta}$ sxa2 ${Delta}$ tip1YFP dhc1GFP cells were treated with pheromone for 5 hours. Cells were visualised with a confocal microscope using a CFP-YFP filter set which allows to separate the YFP and GFP signals. A single plane through the centre of the cells was taken. Arrows indicate fluorescence at the SPB and arrowheads indicate fluorescence at the microtubular tip. Bars, 10 µm (A,B); 3 µm (in C for C and D; F); 5 µm (E).

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