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Fig. 9. Microtubular dynamics have lost coordination in ssm4{Delta} tip1{Delta} cells. (A,B,C) cyr1{Delta} sxa2{Delta} tip1{Delta} nmt1atb2GFP and (D, E) cyr1{Delta} sxa2{Delta} tip1{Delta} ssm4{Delta} nmt1atb2GFP cells were treated with pheromone for 5 hours. (A,D) Cells were then imaged every 6 seconds on a confocal microscope. Images shown are projections of sections through the whole cell. Yellow arrows mark the position of the SPB. + and – indicate growing and shrinking microtubules respectively. (B,C,E) Microtubule lengths for single cells were measured distinguishing the side of the cell in which they lie. B describes the dynamics of the microtubules of the cell in A, diagram E corresponds to D, and diagram in C describes a different tip1{Delta} cell. The coordination of dynamics between opposite cell ends was then calculated as a percentage of total time points; eight cells were scored for a total imaging time of 45 minutes. Bar, 2 µm.





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