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Fig. 1. Nek2 expression in mouse early embryos. (A) RT-PCR analysis. Total RNA isolated from mouse testis, oocytes and embryos at indicated stages was reverse-transcribed followed by PCR amplification with primers specific to a common region of Nek2A and Nek2B (Nek2), to Nek2A or to Nek2B. GAPDH was detected as a control. (B) Detection of the Nek2A and Nek2B proteins. Immunoblot analysis was carried out with lysates from the mouse testis, the human 293T cell line and 293T cells transfected with pNek2RHA1, which encodes the HA-tagged Nek2A protein. Mouse Nek2A protein routinely appeared smaller than the human Nek2A protein. (C) Immunoblot analysis of Nek2 in mouse early embryos. Protein samples were prepared from the mouse testis, oocytes and embryos at indicated developmental stages and were subjected to immunoblot analysis with a polyclonal antibody specific to Nek2. ß-Tubulin was detected as a control.





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