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Fig. 2. Expression and localization of the Nek2 proteins during the first mitosis of the mouse embryo. (A) Mitotic stages of mouse embryos that underwent the first mitosis were determined based on their morphology under a dissecting microscope. I, interphase; P, prophase; M, metaphase; A, anaphase; T, telophase. (B) Mouse embryos were collected based on their mitotic stages and were subjected to immunoblot analysis with the Nek2 antibody. The Nek2A- and Nek2B-specific bands are indicated on the right side of the figure. ß-Tubulin was used as a loading control. (C) A zygote at metaphase was co-immunostained with antibodies specific to Nek2 and {gamma}-tubulin. DNA was stained with DAPI. (D) Immunostaining of the Nek2 protein during the first mitosis of the mouse embryos. Zygotes that were about to undergo the first mitotic division were immunostained with the Nek2 antibody. DNA was stained with DAPI. Arrowheads indicate spindle poles and arrow indicates the mid body.





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