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Fig. 3. Suppression of Nek2 expression with RNAi in mouse early embryos. (A) Specific suppression of Nek2 and E-Cadherin mRNA with RNAi. Double-stranded RNAs specific to Nek2 or E-Cadherin were injected into zygote embryos. The GFP dsRNA was also injected as a non-specific control. After culture for the indicated times, the embryos were subjected to RT-PCR for detection of levels of Nek2 and E-Cadherin mRNA. GAPDH mRNA was detected as a control for RT-PCR. (B) Suppression of Nek2 and E-Cadherin expression with RNAi. Competitive PCR was used to determine suppression levels. A fixed amount of the insertional mutant DNA fragments (Mt) specific to Nek2 or E-Cadherin was added to the PCR reaction mixture along with a variable amount of the reverse-transcribed cDNA (Wt). GAPDH mRNA was detected as a control for the RT-PCR reaction.





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