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Fig. 5. Effects of dsRNA specific to Nek2 and E-Cadherin on mouse early embryo development in culture. (A) One-cell embryos injected with dsRNA specific to GFP, E-Cadherin or Nek2 were cultured for 4 days and the number of embryos reaching the blastocyst stage was counted. Uninjected and vehicle-injected groups were included as controls. Experiments were repeated five times. Data are presented as mean±s.e.m. The number of embryos used for each experimental group is indicated within the bar. (B) Four kinds of Nek2 dsRNAs (see Fig. 4A) were injected into mouse one-cell embryos. Four days later, the number of embryos reaching the blastocyst stage was counted. GFP dsRNA was used as a control. Experiments were repeated five times and data are presented as mean±s.e.m. The number of embryos used for each experimental group is indicated within the bar.
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