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Fig. 7. Mitotic abnormalities in the Nek2-suppressed mouse embryos. (A) Control and Nek2-suppressed embryos were immunostained with antibodies specific to ß-tubulin and {gamma}-tubulin. Nuclei were stained with DAPI. Arrowheads indicate typical MTOCs observed in the control embryo. (B) The number of mitotic blastomeres was counted in uninjected embryos and in embryos injected with dsRNA specific to GFP, Nek2A or Nek2B and the proportions calculated. The experiments were repeated five times and data are presented as mean±s.e.m. The number of embryos used for each experimental group is indicated within the bar. (C) Nuclei of the Nek2-suppressed embryos (b,c) were stained with DAPI and compared with those of the uninjected control embryos (a). In the Nek2-suppressed embryos, abnormal segregations were observed such as dumbbell-like nuclei (arrows) and micronuclei (arrowheads). Nuclei of the polar body are indicated with open arrowheads.





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