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Fig. 10. GW182 is the GWB `matrix/scaffold' protein as revealed in gene knockdown experiments. GW182 (A) and LSm4 (B) in GWBs are detected by staining with respective antibodies and Alexa Fluor 555-conjugated secondary antibody (red; i,v). GW182 siRNA construct-transfected cells are marked by green fluorescent protein, via cotransfection with phrGFP, which is detected both in the nucleus and cytoplasm (ii,vi). GWBs are detected in non-transfected cells (arrows, i,v) and cells transfected with pSHAG vector control (arrowheads, v,viii) but are absent in cells with the siRNA construct (arrowheads, i,iv). (iv,viii) Merged images. Nuclei are counterstained with DAPI (iii,vii). Scale bar: 10 µm.





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