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Fig. 11. HeLa cells selected for GW182 gene knockdown demonstrated a reduction in GW182 protein levels and few or no GWBs. (A) Western blot analysis of GW182 expression using the index human anti-GW182 serum in GW182-knockdown cells (721A9), control selected cells (721B9), and control untreated HeLa cells. Tubulin antibody was used to check for equal loading of samples. Cell lysates were run on a 10% polyacrylamide gel and transferred to nitrocellulose prior to immunoblotting. (B) GW182 and LSm4 in GWBs were detected by staining with respective antibodies and Alexa Fluor-conjugated secondary antibodies (i-iv). Arrowheads in ii and iv indicate 721A9 cells with few or no GWBs; arrows indicate cells with normal GWB expression (i,iii), and reduced GWB expression (ii,iv). Scale bar: 10 µm.

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