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Fig. 2. Immunogold electron microscopy localization of GWBs in the cytoplasm of HeLa cells during interphase. Frozen sections of fixed and gelatin-embedded HeLa cells were incubated with the index human anti-GW182 serum diluted 1:400 and then post-immunolabeled with protein A-gold (10 nm). (A-E) Representative gold-labeled cytoplasmic structures with diameters which vary from 100-300 nm. The gold labels are clustered on electron dense fibrils or strands, 8 to 10 nm in diameter, which are more obvious in some images (arrowheads in A,B,D,E). These fibrils appear to form the matrix that the gold decorates. Some gold particles are observed on the periphery of the cluster (arrows in A,C) while the majority of the gold is found clearly within the center of these structures.
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