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Fig. 7. Increased expression of GWBs and GW182 in proliferating 3T3 cells after release from serum starvation. (A) Cells were analyzed by IIF at different time points after refeeding, as indicated. At 0 hours, there was little or no staining from human anti-GW182 serum. Smaller discrete GWBs (arrows) were observed beginning at 3 and 6 hours after refeeding. Larger and more numerous GWBs were observed at the subsequent time points (arrowheads). Scale bar: 10 µm. (B) Cells were harvested at different time points and supernatant fractions were separated in a 5% gel by SDS-PAGE and transferred to a nitrocellulose membrane that was later probed with a human anti-GW182 serum.





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