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Fig. 8. Enhanced expression of GW182 in proliferating mouse splenocytes and isolated T cells. Isolated mouse spleen cells or purified T cells were cultured in vitro either in the presence or absence of 5µg/ml ConA for 2 to 4 days. (A-D) Representative anti-GW182 IIF images of isolated T cells from day 3. Cells treated with ConA (C) showed cell aggregation and abundant small (arrowheads) and large (arrows) GWBs. Untreated cells were negative for GW182 (A). (B,D) DAPI counterstaining of the cell nuclei shown in A and C, respectively. Scale bar: 10 µm. (E) Western blot comparison of GW182 expression in cells treated with or without ConA.





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