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Fig. 3. No nuclear leakage occurs in PMA-induced pro-apoptotic cells. (A) Electron micrograph of D2 cells that were treated with 32.4 nM PMA for 2 hours in the presence of 50 µM zVAD-fmk. Cells were then washed with PBS and fixed with 4% paraformaldehyde and 1% glutaraldehyde at 4°C for overnight. N, nucleus; C, cytoplasm; a, heterochromatin; b, nuclear envelope; c, nuclear pore complexes. (B) Western blot of the cytosolic and nuclear fractions, as described in the legend to Fig. 2C, with antibodies against hnRNP C1/C2, hnRNP A2/B1, lamin A/C and TK1.
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