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Fig. 5. Expression of the dominant active form of ROCK(CAT) induces cytosolic translocation of hnRNP C1/C2. (A) HEK293T cells on 35 mm culture dishes were transfected with 1 µg of expression plasmid of ROCK(CAT) or control vector overnight. Cells were fixed and stained with antibodies against hnRNP C1/C2 and c-myc, followed by visualization with TRITC-conjugated anti-goat and FITC-conjugated anti-mouse antibodies. Nuclear staining was performed with DAPI. (B) The cytosolic and nuclear fractions from cells as described in A were prepared for western blot analysis with antibodies against hnRNP C1/C2 and hnRNP A2/B1. (C) HEK293T cells were co-transfected with 0.5 µg of expression plasmid of GFP-RhoAV14 or GFP-RhoAV14 E40L or pEGFP and 0.5 µg of pDsRed-hnRNP C1. Cells were stained with Hoechst and observed by fluorescence microscopy.
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