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Fig. 7. The C-terminal 40 amino acids confer nuclear protein ROCK-responsive translocation. (A) HEK293T cells were transfected with 1 µg of pYFP-Nuc or pYFP-Nuc-C40 in combination with 0.5 µg of ROCK(CAT) expression plasmid or control vector as indicated. Cells were fixed and stained with DAPI and observed by fluorescence microscopy for nuclear and YFP expression. (B) The amino acid sequence of the C-terminal 40 amino acids of hnRNP C1 (residues 251-290).





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