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Fig. 1. Characteristics of the lentiviral vectors. The packaging vector, was derived from the prototypic FIV-14-Petaluma genome (AIDS Research and Reference Reagent Program), in which the 5' LTR is replaced by a CMV promoter and the 3' LTR by a poly-A signal. The transfer vector was also obtained from FIV-14-Petaluma by replacing the U3 region of the 5' LTR with a CMV promoter in order to enable the expression of the vector in cells. Moreover, the gag gene was truncated at position 1243 and a frameshift introduced at position 926. The pol, vif, orfA, env and rev genes were replaced by a cassette containing the mutated PrP sequences driven by the phosphoglycerate kinase promoter. The PrP sequence is followed by an IRES sequence and the enhanced GFP marker gene. The third vector pMD.G, used for pseudotyping, contains the VSV-G envelope gene, which encodes the envelope G protein of the vesicular stomatitis virus. PGK, phosphoglycerate kinase promoter; CTE, constitutive transport element; cPPT CTS, central DNA flap sequence; SD, splice donor site.

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