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Fig. 1. (A) RT-PCR analysis of the expression of JAM-1-encoding mRNA in egg and embryo stages. The 600 bp cDNA product is indicated by arrows. (Left) Produced using poly(A)+ RNA with five eggs or embryos per sample. (Right) Total RNA using one egg or embryo equivalent per sample. `Co' indicates the control without the reverse transcription step. (B) Immunoblot analysis of JAM-1 protein expression in eggs and embryos using BV20 antibody, showing approximately 500 eggs or embryos per lane. Mouse lung tissue (18 µg protein) was used as positive tissue control (TC). The stages are unfertilized eggs (UF); fertilized eggs (FE) and embryos at two-cell (2C), four-cell (4C), precompact eight-cell (8C), compact eight-cell (C8), early morula (EM), late morula (LM), early blastocyst (EB) and late blastocyst (LB) stages. AbC, secondary-antibody-only control.





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