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Fig. 6. Mitochondrial TR3 contributes to apoptosis of MGC80-3 cells. (A) Colocalization of mitochondrial TR3 with apoptotic cells. MGC80-3 cells were transfected with GFP-RXR alone or GFP-TR3 together with RXR, and then treated with 9-cis retinoic acid (1 µM) for 48 hours. The nuclear morphology stained by DAPI was visualized using a fluorescence microscope and the two images were merged. Apoptotic cells were indicated by arrows. (B) Effect of TR3 and its mutants on apoptosis induction. MGC80-3 cells were transfected with RXR either alone or with GFP-TR3, GFP-TR3N or GFP-TR3C, and then treated with or without 9-cis-RA (1 µM) for 48 hours. The cells were stained with DAPI to show nuclear morphology. Apoptotic cells that displayed nuclear condensation and fragmentation were scored by examination of 300 transfected cells.

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