QUICK SEARCH:

[advanced]

	Author:		Keyword(s):

Home Help Feedback Subscriptions Archive Search Table of Contents

	Help viewing high resolution images
	Return to article

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.

Fig. 8. RXR ${alpha}$ -TR3 interaction correlates well with their ability to target to the mitochondria. (A) Cellular localization of GFP-TR3 ${Delta}$ N and GFP-TR3 ${Delta}$ C in response to 9-cis-RA. GFP-TR3 ${Delta}$ N or GFP-TR3 ${Delta}$ C together with RXR ${alpha}$ was transfected into MGC80 cells. Cells were treated with or without 9-cis-RA (1 µM) for 12 hours, then immunostained with anti-Hsp60 antibody. GFP-TR3 ${Delta}$ N, GFP-TR3 ${Delta}$ C and mitochondria (Hsp60) were visualized using a confocal microscope. (B) Interaction of RXR ${alpha}$ with different TR3 mutants. GFP-TR3 ${Delta}$ N or GFP-TR3 ${Delta}$ C together with Myc-RXR ${alpha}$ was cotransfected into HEK293 cells. Cell extracts were prepared and immunoprecipitated with anti-Myc antibody. The immunoprecipitate was subjected to SDS-PAGE, blotted and probed with anti-GFP antibody. The same membrane was also blotted with anti-Myc antibody to determine immunoprecipitation (IP) specificity and efficiency. Input represents 5% of cell lysates used in the IP western blot assay. The empty vector was used as a control.

Return to article